Abstract: The present study was carried out to diagnose immune mediated hemolytic anemia associated with Babesia gibsoni in dogs. A total of 136 dogs suspected for B. gibsoni infection were examined by microscopy and screened by PCR, out of which 64 dogs were positive for B. gibsoni infection. Among 64 dogs, 28 dogs were positive for IMHA by Coombs test, 21 dogs were positive for IMHA by Saline agglutination test and 18 dogs had spherocytosis.
