Physiological and biochemical changes during in vitro germination under salinity stress in green gram (Vigna radiata (L.) R. Wilczek)
Author(s): NC Chovatiya ID, UK Kandoliya, MJ Parmar and Hilay Dudhat
Abstract: In this study, ten genotypes of green gram (V1: GJM 1701, V2: GJM 1714, V3: GJM 1837, V4: GJM 1901, V5: GM 4, V6: GM 6, V7: GM 7, V8: GAM 5, V9: K 851, V10: Meha) investigated the salt toleranc eand salt suceptible under various saline water treatments (T0 to T4 = DW to 8 EC). Different genotypes displayed significant variation in physiological and biochemical characteristics. The experimental results revealed that with increase in salinity levels, greater reduction was observed for physiological and biochemical parameters. All physiological and biochemical parameters were found reduced in all the genotypes studied with more reduction at higher salinity (8 EC) level rather than control (Distilled water) level. In physiological parameters, the salt-tolerant variety (GM 6) showed the highest GP% (97.33%) and STI (76.91%), while GJM 1701 showed the lowest GP% (88%) and STI (64.03%) as compared to other varieties. Higher saline water stress causes a decrease in a number of biochemical parameters, including total chlorophyll content, chlorophyll a, and chlorophyll b. However, the salt-susceptible variety GJM 1701 has the lowest total chlorophyll content and the salt-tolerant variety GM 6 has the highest levels of these biochemical parameters. However, salinity causes an increase in lipid peroxidation, especially in the susceptible (GJM 1701) variety. In summary, it was concluded that the GM-6 cultivar could tolerate saline irrigation water. GM 6 cultivar exhibits superior tolerance criteria and performs better at varying salt levels. In terms of salinity tolerance, the following is the order of green gram varieties: GM 6 > GM 7 > Meha > GAM 5 >K 851 > GM 4 > GJM 1901 > GJM 1714 > GJM 1701.
