Author(s): Dr. Shivangi Jain, Dr. Sachin Jain, Dr. Vidhi Gautam, Dr. Rakhi Vaish, Dr. Payal Jain, Dr. Anil Gattani, Simi Jain and Dr. Astha Moolchandani

Abstract: The study investigated Bisphenol F (BPF)-induced intestinal toxicity in zebrafish and assessed the protective effect of quercetin on sub-acute exposure to BPF. The experiment lasted for 21 days, involving 240 zebrafish randomly assigned to six groups (n=40 per group). Group I served as the control, Group II was exposed to BPF (100 µg/L), Group III received quercetin (50 µg/L), Group IV received quercetin (75 µg/L), Group V was simultaneously treated with BPF (100 µg/L) and quercetin (50 µg/L), and Group VI was simultaneously treated with BPF (100 µg/L) and quercetin (75 µg/L). Behavioural parameters were evaluated on days 0 and 21, including line crossings, time spent in white compartments, crossings between compartments, freezing behaviour, swimming time percentage, mirror biting time percentage, and entries into the upper portion of a novel tank. The BPF-treated group (Group II) showed decreased behavioural activity compared to controls, while Groups V and VI exhibited improved parameters, indicating the protective effects of quercetin. Groups III and V showed increased time spent in the upper tank and reduced erratic movements compared to Group II. In contrast, these behaviours were less pronounced in Groups IV and VI. The results indicated that Quercetin in lower doses protect the intestine from subacute intestinal toxicity, which could potentially be used as a novel therapeutic in future.

DOI: 10.33545/26174693.2024.v8.i7b.1457