Author(s): Sajad Ahmad Sheikh, Saroj Rai, Ajoy Das, Prince Clinton Rava, Bed Singh, Jagpal Jogi and Anand Kumar Yadav

Abstract: Quality semen production remains the main aim of semen processing laboratories. The bacteria most responsible for semen contamination originate from the preputial cavity and urinary tract of bucks that find their way through natural mating or artificially by semen collection. The main aim of the study is to determine the bacterial load in the Preputial cavity of Black Bengal Bucks before and after washing with 0.02% KMnO₄. There was a significant decrease (p<0.01) in bacterial load (39.6%), Staphylococci sp. (23%) and Coliform (25%). After preputial washing mean values (log CFU/ml) of Total plate count, Staphylococci spp count, the Coliform count were reduced to 3.77, 5.12, 1.45 from the initial value of 6.25, 6.70, 1.95, respectively. Hence, 0.02 % KMnO₄ solution can be used to wash the prepuce before routine semen collection from Bucks.

DOI: 10.33545/26174693.2024.v8.i7a.1429