Optimizing growth conditions for shiitake mushroom cultivation in Birbhum, West Bengal: A study on media, temperature, and ph variations
Author(s): C Shanmugaraj, K Saranraj and MK Biswas
Abstract: Shiitake mushroom (Lentinula edodes (Berk.) Pegler) is a valuable edible fungus known for its culinary and medicinal properties. Understanding the influence of media, temperature, and pH variations is crucial for the growth and productivity of L. edodes, offering insights into enhancing cultivation practices and yield optimization. The study investigated the influence of various substrates, temperature conditions, and pH levels on the mycelial growth of L. edodes. Potato Dextrose Agar exhibited the highest mycelial growth rate, whereas Rose Bengal Agar showed the lowest. Among the tested substrates, Potato Dextrose Agar was preferred by all three strains, followed by Malt Extract Agar, Sorghum Meal Agar, Saw Dust Extract Agar, and Czapek Dox Agar. Strain LE-02 demonstrated superior mycelial growth compared to the other strains. Regarding temperature, optimal growth was observed at 25 °C, while growth decreased at 15 °C. Deviations from the optimal temperature of 25 °C resulted in reduced mycelial growth. Regarding pH levels, pH 5.0 supported the highest mycelial growth compared to pH levels of 4.0, 6.0, and 7.0. These findings underscore the importance of substrate composition, temperature management, and pH regulation in optimizing the mycelial growth of L. edodes under laboratory conditions.
